Biochemical Markers of Inflammation, Oxidative Stress and Cardiovascular Risk in Adult Patients With Lower Extremity Atherosclerotic Disease With Diabetes vs Without Diabetes: a Cross-Sectional Study

Principal Investigator: Timothy Lyons, MD, FRCP

Abstract:

Objective:  To gather data on 'the differences in disease-related biochemical markers in adult patients with lower extremity atherosclerotic disease (LEAD) with type 2 diabetes vs non-diabetecs' to estimate variations in proposed measures and sample sizes of the larger planned study.

Specific Aims: 

1.  In atheromatous plaque obtained from lower extremity arteries of adult patients with type 2 diabetes vs non-diabetics, determine the differences among tissue levels and types of biochemical markers, modulators and receptors of inflammation and oxidative stress (eg, nitric oxide synthase and coclooxygenase), and the histologic architecture;

2.  In blood samples of adult patients with LEAD with type 2 diabetes vs non-diabetics, determine the differences among levels and types of biochemical markers, modulators and receptors of inflammation and oxidative stress (eg, nitric oxide synthase and cyclooxygenase) in leukocytes and platelets, and the quantities of 'collagen and thrombin' activated (COAT) platelets;

3.  In the serum and urine of adult patients with LEAD with type 2 diabetes with LEAD with type 2 diabetes vs non-diabetics, determine the differences among levels of biochemical markers of inflammation, oxidative stress (eg, CRP, OL-6, TNF-a, CAP-3), and cardiovascular risk (eg, A1C, lipid profile, urine microalbumin).

Participant Inclusion Criteria:

Participant Compensation:

Contact Information:

Muhammad Firdaus, MD (405) 271-5896